Signalling
FecA*
Part:BBa_K257019:Design
Designed by: Romain Bodinier Group: iGEM09_Paris (2009-10-19)
FecA mutated - constitutive activity of the fec operon
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1290
Illegal BglII site found at 2203 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 89
Design Notes
The original sequence contained 1 Pst1 site and required one additional mutation to obtain its constitutive activity.
Source
K12 genomic DNA